Influenza A/B

Influenza A/B

Rapid Influenza A/B Test Panel - For Nasal Swab Specimen


Influenza A&B is a rapid qualitative in vitro assay that detects influenza type A and B antigens (nucleoprotein) extracted from the respiratory specimens. The test is designed to detect nucleoproteins of Flu-A core subtypes, independent of H and N surface proteins (including H1N1 and H3N2). For professional use only.
KIT STORAGE The Rapid Influenza A/B test kits should be stored at room temperature in the original sealed pouch. The expiration date was determined under normal laboratory conditions. Once opened, the extraction buffer vial remain stable if carefully recapped and stored at room temperature below 30°C or refrigerated.



1.  Insert the sterile swab into nostril which shows the most secretion.
2. Very gently rotate and push the swab until resistance is met at level of the turbinates.
3. Gently rotate the swab against nasal wall for a few times.





1. Put 10 drops Extraction Buffer in the test tube,
2. Place the specimen swab in the tube and swirl it vigorously to mix the reagents for at least 1 minute.
3. Then expunge as much liquid as possible from the swab by pressing and rotating the fiber portion against the wall of the tube. Discard the swab. Mix the contents of the tube by gentle swirling. The mixture is ready to test.



1. Remove the test panel from the foil packet, and place it on a flat, dry surface.
2. Holding the sample dropper above the test panel squeeze 3 drops of the mixed specimen into the sample well (Figure 1). Wait until each drop is absorbed, before adding additional drops. If after the first drop, the drop is not absorbed within 30 seconds, follow it up with 2 buffer drops directly from the buffer bottle, and require no additional specimen drops.
3. As the test begins to work, you will see purple color move across the Result Window in the center of the test panel.
4. Interpret test results at 10 minutes. Do not interpret test results after 15 minutes.
Caution: The above interpretation time is based on reading the test results at room temperature of 15 to 30 oC. If your room temperature is significantly lower than 15 oC, then the interpretation time should be properly increased.


1. A color band will appear at the left section of the Result Window to show that the test is working properly. This band is the Control Band.
2. The right section of the Result Window indicates the test results. If another color band appears at the right section of the Result Window, this band is the Test Band.


The presence of two color bands (“T” band and “C” band) within the result window regardless of which band appears first indicates a positive result in either or both Inf-A/Inf-B panel, indicating positive for Inf-A and/or Inf-B respectively (Figure 2, 3 and 4). Note: Generally, the higher the analyte level in the specimen, the stronger the “T” band color will be. When the specimen analyte level is close to but still within the sensitivity limit of the test, the color of the “T” band will be very faint.


The presence of only one color band within the Result Window indicates a negative result (Figure 5).


If after performing the test, no color bands are visible within the Result Window, the result is considered invalid. Some causes of invalid results are: not following the directions correctly or the test may have deteriorated beyond the expiration date. It is recommended that the specimen be re-tested using a new test kit (Figure 6 and 7).
Note: A positive result will not change once it has been established at 15 minutes. However, in order to prevent any incorrect results, the test result should not be interpreted after 15 minutes.


Control standards are not supplied with this kit; however, it is recommended that a control be tested as good laboratory testing practice.